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李 “기름값 담합은 중대범죄…악덕기업, 대가 곧 알게될 것”
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48세 하지원, 물광피부에 깜짝…나이 비껴간 미모
Sanger sequencing reaction mixtures included purified template DNA, a primer, DNA polymerase, and all four dNTPs. Each reaction also included a radiolabeled ddNTP version of just one of the four nucleotides. Only a small amount of each ddNTP was added, however, to ensure that a fraction of the total DNA fragments produced stopped at each occurrence of that base. As with previous methods, separating fragments via length and performing autoradiography allowed scientists to read the final sequence.。搜狗输入法对此有专业解读